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Inside This Issue Aug/Sept

Posted By Connections Editor, Friday, September 8, 2017

August

CBP/Catenin antagonists: Targeting LSCs’ Achilles heel
Yong-Mi Kim, Eun-Ji Gang, Michael Kahn

Reprogramming acute myeloid leukemia into sensitivity for retinoic-acid-driven differentiation
Noortje van Gils, Han J.M.P. Verhagen, Linda Smit

Reciprocal regulation between hepcidin and erythropoiesis and its therapeutic application in erythroid disorders
Caiyi Wang, Zheng Fang, Zesen Zhu, Jing Liu, Huiyong Chen

With me or against me: Tumor suppressor and drug resistance activities of SAMHD1
Nikolas Herold, Sean G. Rudd, Kumar Sanjiv, Juliane Kutzner, Ida Hed Myrberg, Cynthia B.J. Paulin, Thale Kristin Olsen, Thomas Helleday, Jan-Inge Henter, Torsten Schaller

FLT3 ligand regulates thymic precursor cells and hematopoietic stem cells through interactions with CXCR4 and the marrow niche
Kirsten M. Williams, Amber R. Moore, Philip J. Lucas, Juin Wang, Catherine V. Bare, Ronald E. Gress

HIF-1α-stabilizing agent FG-4497 rescues human CD34+ cell mobilization in response to G-CSF in immunodeficient mice
Bianca Nowlan, Kathryn Futrega, Marion E. Brunck, Gail Walkinshaw, Lee E. Flippin, Michael R. Doran, Jean-Pierre Levesque

D816V mutation in the KIT gene activation loop has greater cell-proliferative and anti-apoptotic ability than N822K mutation in core-binding factor acute myeloid leukemia
Ikuko Omori, Hiroki Yamaguchi, Koichi Miyake, Noriko Miyake, Tomoaki Kitano, Koiti Inokuchi

Development of an assay for cellular efflux of pharmaceutically active agents and its relevance to understanding drug interactions
Benigno C. Valdez, Moustapha Hassan, Borje S. Andersson

 

September

B-cell identity as a metabolic barrier against malignant transformation 

Lai N. Chan, Markus Müschen

TAL1 as a master oncogenic transcription factor in T-cell acute lymphoblastic leukemia
Takaomi Sanda, Wei Zhong Leong

Novel tumor-suppressor function of KLF4 in pediatric T-cell acute lymphoblastic leukemia
Ye Shen, Taylor J. Chen, H. Daniel Lacorazza

Lifelong dietary intervention does not affect hematopoietic stem cell function
Seka Lazare, Albertina Ausema, Aaffien C. Reijne, Gertjan van Dijk, Ronald van Os, Gerald de Haan

A novel dual inhibitor of microtubule and Bruton's tyrosine kinase inhibits survival of multiple myeloma and osteoclastogenesis
Manoj K. Pandey, Krishne Gowda, Shen-shu Sung, Thomas Abraham, Tulin Budak-Alpdogan, Giampolo Talamo, Sinisa Dovat, Shantu Amin

PRKCH regulates hematopoietic stem cell function and predicts poor prognosis in acute myeloid leukemia
Shaina N. Porter, Jeffrey A. Magee

Whole-genome analysis reveals unexpected dynamics of mutant subclone development in a patient with JAK2-V617F-positive chronic myeloid leukemia
Ivan Sloma, Maria Teresa Mitjavila-Garcia, Olivier Feraud, Frank Griscelli, Noufissa Oudrhiri, Sanaa El Marsafy, Emilie Gobbo, Dominique Divers, Alexis Proust, David M. Smadja, Christophe Desterke, Annaick Carles, Yusanna Ma, Martin Hirst, Marco A. Marra, Connie J. Eaves, Annelise Bennaceur-Griscelli, Ali G. Turhan

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Inside this Issue: May/June

Posted By Connections Editor, Tuesday, June 20, 2017

May

Chromatin priming of genes in development: Concepts, mechanisms and consequences
Constanze Bonifer, Peter N. Cockerill

Induced pluripotent stem cell technology: A window for studying the pathogenesis of acquired aplastic anemia and possible applications
Mahmoud I. Elbadry, J. Luis Espinoza, Shinji Nakao

Hemangioblast, hemogenic endothelium, and primitive versus definitive hematopoiesis
Georges Lacaud, Valerie Kouskoff

Predictive value of pretransplantation molecular minimal residual disease assessment by WT1 gene expression in FLT3-positive acute myeloid leukemia
Anna Candoni, Federico De Marchi, Francesca Zanini, Maria Elena Zannier, Erica Simeone, Eleonora Toffoletti, Alexsia Chiarvesio, Michela Cerno, Carla Filì, Francesca Patriarca, Renato Fanin

Androgen receptor expression in mantle cell lymphoma: Potential novel therapeutic implications
Elahe A. Mostaghel, Paul S. Martin, Stephen Mongovin, Shani Frayo, Ailin Zhang, Kerstin L. Edlefsen, Oliver W. Press, Ajay K. Gopal

Use of ubiquitous, highly heterozygous copy number variants and digital droplet polymerase chain reaction to monitor chimerism after allogeneic haematopoietic stem cell transplantation
John B. Whitlam, Ling Ling, Michael Swain, Tom Harrington, Oksana Mirochnik, Ian Brooks, Sara Cronin, Jackie Challis, Vida Petrovic, Damien L. Bruno, Francoise Mechinaud, Rachel Conyers, Howard Slater

Governing roles for Trib3 pseudokinase during stress erythropoiesis
Arvind Dev, Ruth Asch, Edward Jachimowicz, Nicole Rainville, Ashley Johnson, Emily Greenfest-Allen, Don M. Wojchowski

Impact of TET2 deficiency on iron metabolism in erythroblasts
Kyoko Inokura, Tohru Fujiwara, Kei Saito, Tatsuya Iino, Shunsuke Hatta, Yoko Okitsu, Noriko Fukuhara, Yasushi Onishi, Kenichi Ishizawa, Kazuya Shimoda, Hideo Harigae

Shipping mouse bone marrow: Keep it in the bone
Ani E. Papazian, Youmna S. Kfoury, David T. Scadden

 

 

June

Application of vitamin D and vitamin D analogs in acute myelogenous leukemia
Huynh Cao, Yi Xu, Rosalia de Necochea-Campion, David J. Baylink, Kimberly J. Payne, Xiaolei Tang, Christina Ratanatharathorn, Yong Ji, Saied Mirshahidi, Chien-Shing Chen

Role of mTORC1–S6K1 signaling pathway in regulation of hematopoietic stem cell and acute myeloid leukemia
Joydeep Ghosh, Reuben Kapur

The evolving view of the hematopoietic stem cell niche
Isabel Beerman, Tiago C. Luis, Sofie Singbrant, Cristina Lo Celso, Simon Méndez-Ferrer

A defined role for multiple Fanconi anemia gene products in DNA-damage-associated ubiquitination
Winnie Tan, Andrew J. Deans

Rat acute GvHD is Th1 driven and characterized by predominant donor CD4+ T-cell infiltration of skin and gut
Margherita Boieri, Pranali Shah, Dasaradha Jalapothu, Olena Zaitseva, Lutz Walter, Bent Rolstad, Christian Naper, Ralf Dressel, Marit Inngjerdingen

Pharmacological inhibition of LSD1 and mTOR reduces mitochondrial retention and associated ROS levels in the red blood cells of sickle cell disease
Ramasamy Jagadeeswaran, Benjamin A. Vazquez, Muthusamy Thiruppathi, Balaji B. Ganesh, Vinzon Ibanez, Shuaiying Cui, James D. Engel, Alan M. Diamond, Robert E. Molokie, Joseph DeSimone, Donald Lavelle, Angela Rivers

The Calreticulin control of human stress erythropoiesis is impaired by JAK2V617F in polycythemia vera
Mario Falchi, Lilian Varricchio, Fabrizio Martelli, Manuela Marra, Orietta Picconi, Agostino Tafuri, Gabriella Girelli, Vladimir N. Uversky, Anna Rita Migliaccio

Novel roles for podocalyxin in regulating stress myelopoiesis, Rap1a, and neutrophil migration
Pan Li, Aldona A. Karaczyn, Rose McGlauflin, Amanda J. Favreau-Lessard, Edward Jachimowicz, Calvin P. Vary, Kailin Xu, Don M. Wojchowski, Pradeep Sathyanarayana

Targeted deletion of the Hoxa cluster affects B lymphopoiesis through depletion of early lymphoid progenitors
Charles-Étienne Lebert-Ghali, Alexander Thompson, Heather J. Melichar, Janet J. Bijl

 

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Inside this issue: Mar/Apr

Posted By Connections Editor, Friday, April 21, 2017

March

‘Off-the-shelf’ immunotherapy with iPSC-derived rejuvenated cytotoxic T lymphocytes
Miki Ando, Hiromitsu Nakauchi

Preclinical approaches in chronic myeloid leukemia: from cells to systems
Cassie J. Clarke, Tessa L. Holyoake

From the bedside to the bench: new discoveries on blood cell fate and function
Eirini Trompouki, Eugenia Flores-Figueroa, Daniel Lucas, Teresa V. Bowman

Targeting the transcription factor Myb by small-molecule inhibitors
Sagar Uttarkar, Jon Frampton, Karl-Heinz Klempnauer

Immunogenicity of murine mPEG-red blood cells and the risk of anti-PEG antibodies in human blood donors
Yevgeniya Le, Wendy M. Toyofuku, Mark D. Scott

Everolimus restrains the IL-17A-dependent osteoclast-like transdifferentiation of dendritic cells in multiple myeloma
Marco Tucci, Stefania Stucci, Anna Passarelli, Stella D'Oronzo, Franco Silvestris

Variable aldehyde dehydrogenase activity and effects on chemosensitivity of primitive human leukemic cells
Anja Bogen, Christian Buske, Wolfgang Hiddemann, Stefan K. Bohlander, Oliver Christ

MLL-AF4 binds directly to a BCL-2 specific enhancer and modulates H3K27 acetylation
Laura Godfrey, Jon Kerry, Ross Thorne, Emmanouela Repapi, James O.J. Davies, Marta Tapia, Erica Ballabio, Jim R. Hughes, Huimin Geng, Marina Konopleva, Thomas A. Milne

Mysm1 expression in the bone marrow niche is not essential for hematopoietic maintenance
Jessica C. Petrov, Anastasia Nijnik

MOZ and BMI1 act synergistically to maintain hematopoietic stem cells
Bilal N. Sheikh, Donald Metcalf, Anne K. Voss, Tim Thomas

April

Donor lymphocyte infusions in AML and MDS: Enhancing the graft-versus-leukemia effect
Guillermo Orti, Pere Barba, Laura Fox, Olga Salamero, Francesc Bosch, David Valcarcel

Polycomb complexes PRC1 and their function in hematopoiesis
Miguel Vidal, Katarzina Starowicz

Kinase signaling and targeted therapy for primary myelofibrosis
Qiong Yang, John D. Crispino, Qiang Jeremy Wen

Mutational analysis of hypermutation-related POLE gene in acute leukemias and lymphomas
Eun Ji Choi, Min Sung Kim, Nam Jin Yoo, Sug Hyung Lee

Analysis of parameters that affect human hematopoietic cell outputs in mutant c-kit-immunodeficient mice
Paul H. Miller, Gabrielle Rabu, Margarita MacAldaz, David J.H.F. Knapp, Alice M.S. Cheung, Kiran Dhillon, Naoto Nakamichi, Philip A. Beer, Leonard D. Shultz, R. Keith Humphries, Connie J. Eaves

Ixazomib suppresses human dendritic cell and modulates murine graft-versus-host disease in a schedule-dependent fashion
Ahmad Samer Al-Homsi, Austin Goodyke, Kelli Cole, Marlee Muilenburg, Michael McLane, Sarah Abdel-Mageed, Yuxin Feng

The BAF45a/PHF10 subunit of SWI/SNF-like chromatin remodeling complexes is essential for hematopoietic stem cell maintenance
Veneta Krasteva, Gerald R. Crabtree, Julie A. Lessard

EphA5 and EphA7 forward signaling enhances human hematopoietic stem and progenitor cell maintenance, migration, and adhesion via Rac1 activation
Thao M. Nguyen, Agnieszka Arthur, Andrew C.W. Zannettino, Stan Gronthos

Impairment of fetal hematopoietic stem cell function in the absence of Fancd2
Sakiko Suzuki, Ronny R. Racine, Nathan A. Manalo, Sharon B. Cantor, Glen D. Raffel

 

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Inside this issue: Jan/Feb

Posted By Connections Editor, Tuesday, February 28, 2017

January

 

Using zebrafish models of leukemia to streamline drug screening and discovery
Adam P. Deveau, Victoria L. Bentley, Jason N. Berman

Novel strategies of adoptive immunotherapy: How natural killer cells may change the treatment of elderly patients with acute myeloblastic leukemia
Roberto M. Lemoli, Sarah Parisi, Antonio Curti

Tumor necrosis factor α in the onset and progression of leukemia
Xiaoxi Zhou, Zhuoya Li, Jianfeng Zhou

Clinical-grade regulatory T cells: Comparative analysis of large-scale expansion conditions

Sarvari Velaga, Christina Alter, Ulrike Dringenberg, Christina T. Thiesler, Sandra Kuhs, Sven Olek, Sya N. Ukena, Anke Franzke

AC220 and AraC cause differential inhibitory dynamics in patient-derived M5-AML with FLT3-ITD and, thus, ultimately distinct therapeutic outcomes
Xiaoyu An, Jinping Liu, Na Wang, Di Wang, Liang Huang, Likun Zhang, Jie Cai, Jean-Pierre Wery, Demin Zhou, Jianfeng Zhou, Qi-Xiang Li

Constitutive Kit activity triggers B-cell acute lymphoblastic leukemia-like disease in mice
Robin R. Weidemann, Rayk Behrendt, Kristina B. Schoedel, Werner Müller, Axel Roers, Alexander Gerbaulet

Alterations in the bone marrow microenvironment may elicit defective hematopoiesis: a comparison of aplastic anemia, chronic myeloid leukemia, and normal bone marrow
Meerim Park, Chan-Jeoung Park, Young Wook Cho, Seongsoo Jang, Jung-Hee Lee, Je-Hwan Lee, Kyoo-Hyung Lee, Young Ho Lee

The AMP-activated protein kinase beta 1 subunit modulates erythrocyte integrity
Emma L. Cambridge, Zoe McIntyre, Simon Clare, Mark J. Arends, David Goulding, Christopher Isherwood, Susana S. Caetano, Carmen Ballesteros Reviriego, Agnieszka Swiatkowska, Leanne Kane, Katherine Harcourt, The Sanger Mouse Genetics Project, David J. Adams, Jacqueline K. White, Anneliese O. Speak

Sustained enhancement of OCTN1 transporter expression in association with hydroxyurea induced γ-globin expression in erythroid progenitors
Aisha L. Walker, Solomon F. Ofori-Acquah

Pharmacological inhibition of AKT activity in human CD34+ cells enhances their ability to engraft immunodeficient mice
Sisi Chen, Rui Gao, Michihiro Kobayashi, Hao Yu, Chonghua Yao, Reuben Kapur, Mervin C. Yoder, Yan Liu

Proinflammatory signals are insufficient to drive definitive hematopoietic specification of human HSCs in vitro

Alessandra Giorgetti, Julio Castaño, Clara Bueno, Rafael Díaz de la Guardia, Mario Delgado, Anna Bigas, Lluis Espinosa, Pablo Menendez

 

February


Ikaros: Exploiting and targeting the hematopoietic stem cell niche in B-progenitor acute lymphoblastic leukemia
Michelle L. Churchman, Charles G. Mullighan

Granulocyte colony-stimulating factor receptor signaling in severe congenital neutropenia, chronic neutrophilic leukemia, and related malignancies
Pankaj Dwivedi, Kenneth D. Greis

Transcriptional activation by MLL fusion proteins in leukemogenesis
Akihiko Yokoyama

Dynamic variation of CD5 surface expression levels within individual chronic lymphocytic leukemia clones
Rachael J.M. Bashford-Rogers, Anne L. Palser, Clare Hodkinson, Joanna Baxter, George A. Follows, George S. Vassiliou, Paul Kellam

Genetically re-engineered K562 cells significantly expand and functionally activate cord blood natural killer cells: Potential for adoptive cellular immunotherapy

Janet Ayello, Jessica Hochberg, Allyson Flower, Yaya Chu, Laxmi V. Baxi, William Quish, Carmella van de Ven, Mitchell S. Cairo

Lenalidomide enhances the function of dendritic cells generated from patients with multiple myeloma
Manh-Cuong Vo, Truc Anh-NguyenThi, Hyun-Ju Lee, Thanh-Nhan Nguyen-Pham, Thangaraj Jaya Lakshmi, Sung-Hoon Jung, Hyeoung-Joon Kim, Je-Jung Lee

MiR-17-92 represses PTPROt and PP2A phosphatases and amplifies tonic BCR signaling in DLBCL cells
Ewa Jablonska, Patryk Gorniak, Maciej Szydlowski, Tomasz Sewastianik, Emilia Bialopiotrowicz, Anna Polak, Krzysztof Warzocha, Przemyslaw Juszczynski

AML1–ETO promotes SIRT1 expression to enhance leukemogenesis of t(8;21) acute myeloid leukemia
Lei Zhou, Qian Wang, Xiaosu Chen, Lin Fu, Xiaodong Zhang, Lijun Wang, Ailing Deng, Dandan Li, Jing Liu, Na Lv, Lili Wang, Yonghui Li, Daihong Liu, Li Yu, Liping Dou

Csk-binding protein controls red blood cell development via regulation of Lyn tyrosine kinase activity
Janice H.C. Plani-Lam, Neli S. Slavova-Azmanova, Nicole Kucera, Alison Louw, Jiulia Satiaputra, Peter Singer, Kong-Peng Lam, Margaret L. Hibbs, Evan Ingley

HIF1α-induced PDGFRβ signaling promotes developmental HSC production via IL-6 activation
Sung-Eun Lim, Virginie Esain, Wanda Kwan, Lindsay N. Theodore, Mauricio Cortes, Isaura M. Frost, Sarah Y. Liu, Trista E. North

Erratum to “Effect of roflumilast, novel phosphodiesterase-4 inhibitor, on lung chronic graft-versus-host disease in mice”
Sei Won Kim, Ji Young Lim, Chin Kook Rhee, Ji Hye Kim, Chan Kwon Park, Tae Jung Kim, Chul Soo Cho, Chang Ki Min, Hyoung Kyu Yoon

Erratum to “Autophagy regulates the cell cycle of mouse HSPCs in a nutrient-dependent manner”
Yan Cao, Aihong Zhang, Jinyang Cai, Na Yuan, Weiwei Lin, Shengbing Liu, Fei Xu, Lin Song, Xin Li, Yixuan Fang, Zhen Wang, Zhijian Wang, Jian Wang, Han Zhang, Wenli Zhao, Shaoyan Hu, Suping Zhang, Jianrong Wang

 

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Inside this Issue: Nov/Dec

Posted By Connections Editor, Wednesday, December 14, 2016

November

Delivery of RNA-based molecules to human hematopoietic stem and progenitor cells for modulation of gene expression 

Yvonne Diener, Andreas Bosio, Ute Bissels

Mechanisms of heparanase inhibitors in cancer therapy
Benjamin Heyman, Yiping Yang


Improving the safety of T-Cell therapies using an inducible caspase-9 gene
Xiaoou Zhou, Malcolm K. Brenner

Precision in donor selection: Identifying ideal stem-cell donors through their T cells
Adam Widman, Ran Reshef

Longitudinal analyses of leukemia-associated antigen-specific CD8+ T cells in patients after allogeneic stem cell transplantation
Elke Rücker-Braun, Cornelia S. Link, Maria Schmiedgen, Antje Tunger, Petra Vizjak, Raphael Teipel, Rebekka Wehner, Denise Kühn, Yannik F. Fuchs, Uta Oelschlägel, Lothar Germeroth, Marc Schmitz, Martin Bornhäuser, Johannes Schetelig, Falk Heidenreich

A genome-wide association study by ImmunoChip reveals potential modifiers in myelodysplastic syndromes
Fabrice Danjou, Claudio Fozza, Magdalena Zoledziewska, Antonella Mulas, Giovanna Corda, Salvatore Contini, Fausto Dore, Antonio Galleu, Anna Angela Di Tucci, Giovanni Caocci, Eleonora Gaviano, Giancarlo Latte, Attilio Gabbas, Paolo Casula, Lucia Gemma Delogu, Giorgio La Nasa, Emanuele Angelucci, Francesco Cucca, Maurizio Longinotti

Complement cascade gene expression defines novel prognostic subgroups of acute myeloid leukemia

Isabelle Laverdière, Meaghan Boileau, Tobias Herold, Janusz Rak, Wolfgang E. Berdel, Bernhard Wörmann, Wolfgang Hiddemann, Karsten Spiekermann, Stefan K. Bohlander, Kolja Eppert

JAK2 V617F stimulates proliferation of erythropoietin-dependent erythroid progenitors and delays their differentiation by activating Stat1 and other nonerythroid signaling pathways
Jiahai Shi, Bingbing Yuan, Wenqian Hu, Harvey Lodish

Quantitative proteomic analysis and comparison of two bone marrow stromal cell lines using the SILAC method
Xiang Li, Ting Wan, Sijie Zhang, Dongliang Li, Xiaofeng Han

Human blood cell levels of 5-hydroxymethylcytosine (5hmC) decline with age, partly related to acquired mutations in TET2

Manuel Buscarlet, Alain Tessier, Sylvie Provost, Luigina Mollica, Lambert Busque

Uncoupling protein 2 deficiency results in higher neutrophil counts and lower B-cell counts during aging in mice
Christin Kretzschmar, Catrin Roolf, Katrin Timmer, Anett Sekora, Gudrun Knübel, Hugo Murua Escobar, Robert Jaster, Sarah Müller, Georg Fuellen, Rüdiger Köhling, Christian Junghanss

Tunneling nanotubes mediate the transfer of stem cell marker CD133 between hematopoietic progenitor cells
Doreen Reichert, Julia Scheinpflug, Jana Karbanová, Daniel Freund, Martin Bornhäuser, Denis Corbeil

 

December

NOX-driven ROS formation in cell transformation of FLT3-ITD-positive AML 

Ashok Kumar Jayavelu, Jennifer N. Moloney, Frank-D. Böhmer, Thomas G. Cotter 

Variegated RHOA mutations in human cancers 
Keisuke Kataoka, Seishi Ogawa 

Translating leukemia stem cells into the clinical setting: Harmonizing the heterogeneity 
Breann Yanagisawa, Gabriel Ghiaur, B. Douglas Smith, Richard J. Jones 
 
Preclinical rationale for TGF-β inhibition as a therapeutic target for the treatment of myelofibrosis 
Ilaria Ceglia, Amylou C. Dueck, Francesca Masiello, Fabrizio Martelli, Wu He, Giulia Federici, Emanuel F. Petricoin III, Ann Zeuner, Camelia Iancu-Rubin, Rona Weinberg, Ronald Hoffman, John Mascarenhas, Anna Rita Migliaccio 
 
Metformin inhibits JAK2V617F activity in MPN cells by activating AMPK and PP2A complexes containing the B56α subunit 
Ichiro Kawashima, Keita Kirito 
 
MicroRNA-155 is upregulated in MLL-rearranged AML but its absence does not affect leukemia development 
Edith Schneider, Anna Staffas, Linda Röhner, Kathrin Krowiorz, Michael Heuser, Konstanze Döhner, Lars Bullinger, Hartmut Döhner, Linda Fogelstrand, Arefeh Rouhi, Florian Kuchenbauer, Lars Palmqvist 
 
Immunological properties of bone marrow microenvironment 1 year after allogeneic hematopoietic stem cell transplantation 
Agnieszka Ciomber, Iwona Mitrus, Wojciech Fidyk, Andrzej Smagur, Agata Chwieduk, Magdalena Glowala-Kosinska, Tomasz Czerw, Małgorzata Sobczyk-Kruszelnicka, Włodzimierz Mendrek, Maria Sadus-Wojciechowska, Jacek Najda, Jerzy Holowiecki, Sebastian Giebel 
 
Primary cilia are present on human blood and bone marrow cells and mediate Hedgehog signaling 
Mohan Singh, Parvesh Chaudhry, Akil A. Merchant 
 
Loss of ASXL1 triggers an apoptotic response in human hematopoietic stem and progenitor cells 
Susan Hilgendorf, Hendrik Folkerts, Jan Jacob Schuringa, Edo Vellenga 

 

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Inside this Issue: September/October

Posted By Connections Editor, Tuesday, November 1, 2016

Pentraxin-3 levels in graft-versus-host disease during allogeneic hematopoietic stem cell transplantation

 Jan-Moritz Doehn, Andreas Winkler, Zoya Kuzmina, Anastasiya Hladik, Hildegard Greinix, Sylvia Knapp, Oliver Robak

The spleen dictates platelet destruction, anti-platelet antibody production, and lymphocyte distribution patterns in a murine model of immune thrombocytopenia
Rukhsana Aslam, Rick Kapur, George B. Segel, Li Guo, Anne Zufferey, Heyu Ni, John W. Semple

Clinical significance and origin of leukocytes that lack HLA-A allele expression in patients with acquired aplastic anemia
Hiroyuki Maruyama, Takamasa Katagiri, Koichi Kashiwase, Takashi Shiina, Aiko Sato-Otsubo, Yoshitaka Zaimoku, Kana Maruyama, Kohei Hosokawa, Ken Ishiyama, Hirohito Yamazaki, Hidetoshi Inoko, Seishi Ogawa, Shinji Nakao

Effects of GlyT1 inhibition on erythropoiesis and iron homeostasis in rats
Michael Winter, Jürgen Funk, Annette Körner, Daniela Alberati, Francois Christen, Georg Schmitt, Bernd Altmann, Andreas Pospischil, Thomas Singer

Characterization of memory B cells from thymus and its impact for DLBCL classification
Kim Steve Bergkvist, Martin Agge Nørgaard, Martin Bøgsted, Alexander Schmitz, Mette Nyegaard, Michael Gaihede, John Bæch, Marie-Louise Grønholdt, Frank Svendsen Jensen, Preben Johansen, Thomas Urup, Tarec C. El-Galaly, Jakob Madsen, Julie Støve Bødker, Karen Dybkær, Hans Erik Johnsen

Alterations of circulating lymphoid committed progenitor cellular metabolism after allogeneic stem cell transplantation in humans
Salomé Glauzy, Régis Peffault de Latour, Isabelle André-Schmutz, Joël Lachuer, Sophie Servais, Gérard Socié, Emmanuel Clave, Antoine Toubert

Calreticulin mutation does not contribute to disease progression in essential thrombocythemia by inhibiting phagocytosis
Shinya Daitoku, Katsuto Takenaka, Takuji Yamauchi, Ayano Yurino, Fumiaki Jinnouchi, Takuya Nunomura, Tetsuya Eto, Tomohiko Kamimura, Masakazu Higuchi, Naoki Harada, Noriyuki Saito, Toshihiro Miyamoto, Hiromi Iwasaki, Koichi Akashi

Spliceosomal component Sf3b1 is essential for hematopoietic differentiation in zebrafish
Adriana De La Garza, Rosannah C. Cameron, Sara Nik, Sara G. Payne, Teresa V. Bowman

Overexpression of LMO2 causes aberrant human T-Cell development in vivo by three potentially distinct cellular mechanisms
Anna-Sophia Wiekmeijer, Karin Pike-Overzet, Martijn H. Brugman, Marja C.J.A. van Eggermond, Martijn Cordes, Edwin F.E. de Haas, Yunlei Li, Edwin Oole, Wilfred F.J. van IJcken, R. Maarten Egeler, Jules P. Meijerink, Frank J.T. Staal

A novel lymphoid progenitor cell population (LSKlow) is restricted by p18INK4c

Fang Dong, Sha Hao, Shihui Ma, Hui Cheng, Yajie Wang, Wen Zhou, Weiping Yuan, Hideo Ema, Tao Cheng

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Inside this Issue: July/August

Posted By Connections Editor, Friday, August 19, 2016
Overexpression of LMO2 causes aberrant human T-Cell development in vivo by three potentially distinct cellular mechanisms
Anna-Sophia Wiekmeijer, Karin Pike-Overzet, Martijn H. Brugman, Marja C.J.A. van Eggermond, Martijn Cordes, Edwin F.E. de Haas, Yunlei Li, Edwin Oole, Wilfred F.J. van IJcken, R. Maarten Egeler, Jules P. Meijerink, Frank J.T. Staal

HnRNP K contributes to drug resistance in acute myeloid leukemia through the regulation of autophagy
JinFang Zhang, XiaoLi Liu, YuDeng Lin, YuLing Li, JianWei Pan, Sa Zong, YongKang Li, Yang Zhou

Clonal hematopoiesis as determined by the HUMARA assay is a marker for acquired mutations in epigenetic regulators in older women
Julia Erin Wiedmeier, Catherine Kato, Zhenzhen Zhang, Hyunjung Lee, Jennifer Dunlap, Eric Nutt, Rogan Rattray, Sarah McKay, Christopher Eide, Richard Press, Motomi Mori, Brian Druker, Kim-Hien Dao

Long-term adaptation to hypoxia preserves hematopoietic stem cell function
Jichun Chen, Ju-Gyeong Kang, Keyvan Keyvanfar, Neal S. Young, Paul M. Hwang

A novel lymphoid progenitor cell population (LSKlow) is restricted by p18INK4c
Fang Dong, Sha Hao, Shihui Ma, Hui Cheng, Yajie Wang, Wen Zhou, Weiping Yuan, Hideo Ema, Tao Cheng

Epigenetic therapy as a novel approach for GFI136N-associated murine/human AML
Lacramioara Botezatu, Lars C. Michel, Anne Helness, Charles Vadnais, Hideki Makishima, Judith M. Hönes, François Robert, Lothar Vassen, Aniththa Thivakaran, Yahya Al-Matary, Robert F. Lams, Judith Schütte, Bernd Giebel, André Görgens, Michael Heuser, Hind Medyouf, Jaroslaw Maciejewski, Ulrich Dührsen, Tarik Möröy, Cyrus Khandanpour

Trim33/Tif1γ is involved in late stages of granulomonopoiesis in mice

Marie-Lorraine Chrétien, Caroline Legouge, Romain Z. Martin, Arlette Hammann, Malika Trad, Romain Aucagne, Anne Largeot, Jean-Noël Bastie, Laurent Delva, Ronan Quéré

Biological implications of somatic DDX41 p.R525H mutation in acute myeloid leukemia
Moe Kadono, Akinori Kanai, Akiko Nagamachi, Satoru Shinriki, Jin Kawata, Koji Iwato, Taiichi Kyo, Kumi Oshima, Akihiko Yokoyama, Takeshi Kawamura, Reina Nagase, Daichi Inoue, Toshio Kitamura, Toshiya Inaba, Tatsuo Ichinohe, Hirotaka Matsui

A dimeric peptide with erythropoiesis-stimulating activity uniquely affects erythropoietin receptor ligation and cell surface expression
Rakesh Verma, Jennifer M. Green, Peter J. Schatz, Don M. Wojchowski


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Inside this Issue: May/June

Posted By Connections Editor, Thursday, June 16, 2016

June

Cladribine, gemcitabine, busulfan, and SAHA combination as a potential pretransplant conditioning regimen for lymphomas: A preclinical study
Jie Ji, Benigno C. Valdez, Yang Li, Yan Liu, Esmeralda C. Teo, Yago Nieto, Richard E. Champlin, Borje S. Andersson

  • Cladribine, gemcitabine, busulfan, and SAHA (suberoylanilide hydroxamic acid) exert synergistic effects in lymphoma cell lines.
  • This combination activates DNA damage response and apoptosis.
  • Pre-exposure to SAHA improves drug efficacy.
  • This combination may be used as the basis for a clinical trial of stem cell transplantation for lymphoma patients. 

A preliminary study on epigenetic regulation of Acanthopanax senticosus in leukemia cell lines
Qing-Yuan Wang, Hua Zhong, Fang-Yuan Chen, Min-Yue Zhang, Jia-Yi Cai, Ji-Hua Zhong

  • Acanthopanax senticosus induced apoptosis of human leukemia HL-60 and HL60/ADM cells in a dose- and time-dependent manner.
  • Acanthopanax senticosus promoted histone H3 acetylation by inhibiting histone deacetyltransferase activity.
  • Acanthopanax senticosus induced FasL expression in HL60/ADM cells, probably through histone H3 promotion. 

SENP1, but not fetal hemoglobin, differentiates Andean highlanders with chronic mountain sickness from healthy individuals among Andean highlanders
Matthew M. Hsieh, David Callacondo, Jose Rojas-Camayo, Jose Quesada-Olarte, Xunde Wang, Naoya Uchida, Irina Maric, Alan T. Remaley, Fabiola Leon-Velarde, Francisco C. Villafuerte, John F. Tisdale

  • Higher hemoglobin levels consistently distinguish persons with chronic mountain sickness from healthy persons.
  • Fetal hemoglobin levels of all Andean highlanders (with and without chronic mountain sickness) were similar to the levels of fetal hemoglobin found in persons at sea level.
  • Classic hypoxia inducible factor regulated EPAS1 and EGLN1 were also similar in healthy Andean highlanders and those with chronic mountain sickness.
  • SENP1 may play a role in Andean high-altitude adaptation.

Quantitative analysis of dietary iron utilization for erythropoiesis in response to body iron status
Yukari Matsuo-Tezuka, Mariko Noguchi-Sasaki, Mitsue Kurasawa, Keigo Yorozu, Yasushi Shimonaka 

  • 57Fe is a useful tool in tracing dietary iron.
  • The contribution of dietary iron to erythropoiesis can be quantified by 57Fe.
  • Stored iron is preferentially used for erythropoiesis under conditions of iron loading.
  • Regulation of iron release is tissue specific.

The frequency of multipotent CD133+CD45RA−CD34+ hematopoietic stem cells is not increased in fetal liver compared with adult stem cell sources 

Stefan Radtke, Kevin G. Haworth, Hans-Peter Kiem 

  • Multipotent hematopoietic stem cells are not increased in human fetal liver compared with adult tissues.
  • The lymphomyeloid and erythromyeloid lineages are segregated in fetal hematopoiesis.
  • Fetal liver hematopoietic stem cells/multipotent progenitors are primed for erythromyeloid lineage specification.
  • NOD/SCID mouse engraftment is driven mostly by lymphomyeloid-restricted, lymphomyeloid-primed progenitors. 

Evaluation of GMP-compliant culture media for in vitro expansion of human bone marrow mesenchymal stromal cells
Patrick Wuchter, Marcel Vetter, Rainer Saffrich, Anke Diehlmann, Karen Bieback, Anthony D. Ho, Patrick Horn

  • Xeno-free human platelet lysate-based cell expansion of human bone marrow-derived mesenchymal stromal cells (MSCs) was comprehensively analyzed and directly compared with two commercially available media: StemPro MSC SFM CTS (for human ex vivo tissue and cell culture processing applications) and MSCGM (non-GMP-compliant, for research only).
  • Quantitative and qualitative analysis of the resulting cell populations included assessment of proliferation kinetics, differentiation capacity, cell morphology, and immunophenotype.
  • A blueprint for an MSC-expansion strategy on a clinically relevant scale is presented.
  • Cost analyses for MSC expansion strategies with all three media were assessed.

May

Phagocyte function decreases after high-dose treatment with melphalan and autologous stem cell transplantation in patients with multiple myeloma
Stina Wichert, Åsa Pettersson, Thomas Hellmark, Åsa Johansson, Markus Hansson

  • We examined changes in phagocyte phenotype and function after ASCT in MM patients.
  • PMNs showed reduced capacity for phagocytosis and oxidative burst.
  • Eosinophils were markedly reduced and slow to regenerate.
  • HLA-DR expression by monocytes was significantly depressed.
  • Several aspects of phagocytic functions are affected in MM patients after ASCT.

Leukemia and chromosomal instability in aged Fancc−/− mice
Donna Cerabona, Zejin Sun, Grzegorz Nalepa

  • Aging Fancc−/− mice are prone to hematopoietic malignancies
  • Chromosomal abnormalities in hematopoietic Fancc−/− cells precede the development of leukemia
  • Fancc−/− leukemia can be successfully transplanted into healthy wt recipients 

Expression of surface-associated 82kDa-proMMP-9 in primary acute leukemia blast cells inversely correlates with patients' risk 
Joerg Schmohl, Donato Santovito, Thomas Guenther, Wishnu Sutanto, Tanja Kroell, Helmut Salih, Thomas Pitsch, Virginia Egea, Christian Weber, Helga Schmetzer, Christian Ries 

  • Monoclonal antibodies have been generated that specifically detect a unique cell surface-associated MMP-9 variant.
  • The MMP-9 variant is present in monocytes and acute myeloid leukemia blasts, but is scarce in T cells and B cells.
  • High expression levels of MMP-9 variant in acute myeloid leukemia blasts correlate with a favorable risk for patients. 

 

    Phenotypic, genotypic, and functional characterization of normal and acute myeloid leukemia-derived marrow endothelial cells
    Russell J. Pizzo, Mitra Azadniv, Naxin Guo, Joshua Acklin, Kimberly Lacagnina, Myra Coppage, Jane L. Liesveld

    • Endothelial cells can be cultured from acute myeloid leukemia (AML)-derived marrow, but these grew more slowly and exhibited increased senescence, as measured by β-galactosidase staining, compared with endothelial cells cultured from normal subject-derived marrow.
    • AML-derived marrow endothelial cells expressed endothelial markers and surface adhesion receptors similar to those of normal subject-derived marrow endothelial cells; the AML-derived cells had similar transmigration and clonogenic support capacity, but increased adhesion of leukemia blasts.
    • RNA sequencing revealed 130 genes differentially expressed between AML-derived and normal subject-derived marrow endothelial cells, including significant upregulation of c-fos and CXCL16.

    Insulin-like growth factor 2 modulates murine hematopoietic stem cell maintenance through upregulation of p57 

    Dolly D. Thomas, Andreia Gianotti Sommer, Alejandro B. Balazs, Isabel Beerman, George J. Murphy, Derrick Rossi, Gustavo Mostoslavsky

    • Insulin-like growth factor 2 (IGF2) is preferentially expressed in long-term hematopoietic stem cells (HSCs).
    • Within HSCs, IGF2 activates the PI3K-Akt pathway to upregulate expression of p57.
    • HSCs exhibit decreased methylation at CpG sites within the p57 gene promoter.
    • The described mechanism illustrates a novel regulatory function for IGF2 in HSCs.

     




     

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    Inside this Issue: March/April

    Posted By Connections Editor, Thursday, April 14, 2016
    Updated: Monday, April 11, 2016

    March

    Combination epigenetic and immunotherapy overcomes resistance to monoclonal antibodies in hematologic malignancies: A new therapeutic approach
    Elliot M. Epner, Bikramajit Singh Saroya, Zainul S. Hasanali, Thomas P. Loughran  

    • Cladribine, histone deacetylase inhibitor, and alemtuzumab constitute an effective therapy for T-cell prolymphocytic leukemia.
    • Cladribine and histone deacetylase inhibitor induce CD30 and allow for brentuximab vedotin therapy in T-cell prolymphocytic leukemia.
    • The combination of DNA methyltransferase inhibitor, histone deacetylase inhibitor, and monoclonal antibody therapy is an effective treatment paradigm for cancer.
    • Antibody-dependent cell-mediated cytotoxicity is central to the mechanism of action of monoclonal antibody therapy.

    CD44 promotes chemoresistance in T-ALL by increased drug efflux
    Catherine Hoofd, Xuehai Wang, Sonya Lam, Catherine Jenkins, Brent Wood, Vincenzo Giambra, Andrew P. Weng 

    • Chemotherapy selected for increased CD44 expression in a mouse model of T-cell acute lymphoblastic leukemia (T-ALL).
    • Enforced CD44 expression in mouse and human T-ALL cells enhanced chemoresistance.
    • CD44 knockdown or treatment with blocking antibody enhanced chemosensitivity.
    • CD44 enhanced drug efflux activity in human T-ALL cells.
    • Patients undergoing induction chemotherapy had elevated CD44 levels in residual blasts.

    Inhibition of isoprenylcysteine carboxylmethyltransferase augments BCR-ABL1 tyrosine kinase inhibition-induced apoptosis in chronic myeloid leukemia
    Wen Tian Sun, Wei Xiang, Bee Ling Ng, Kartini Asari, Ralph M. Bunte, Patrick J. Casey, Mei Wang, Charles Chuah 

    • Isoprenylcysteine carboxylmethyltransferase inhibition enhances the effect of BCR-ABL tyrosine kinase inhibition.
    • Cysmethynil, an isoprenylcysteine carboxylmethyltransferase inhibitor, augments tyrosine kinase inhibitor-induced apoptosis in chronic myeloid leukemia cells.
    • The combination of cysmethynil and imatinib inhibits colony formation in primary chronic myeloid leukemia cells.
    • The in vivo effects of the cysmethynil/imatinib combination are enhanced compared with the effects of either drug alone.
    • Enhanced apoptosis may be caused by immediate and persistent inhibition of MAPK signaling.

    Fms-like tyrosine kinase 3 (Flt3) ligand depletes erythroid island macrophages and blocks medullar erythropoiesis in the mouse
    Rebecca N. Jacobsen, Bianca Nowlan, Marion E. Brunck, Valerie Barbier, Ingrid G. Winkler, Jean-Pierre Levesque

    • Daily administration of Flt3 ligand in mice depletes erythroblastic island macrophages and inhibits erythropoiesis in the bone marrow.
    • Suppression of medullary erythropoiesis is compensated by increases in erythroblastic island macrophage numbers and erythropoiesis in the spleen.
    • The suppressive effect of Flt3 ligand on medullary erythroblastic island macrophages and erythropoiesis is likely to be indirect, as
    • Flt3 receptor is not expressed by macrophages or erythroid progenitors.

     

    April

    Ex vivo evaluation of the effect of regulatory T cells on the anti-tumor activity of bortezomib in multiple myeloma
    Ayse Pinar Ercetin, Mehmet Ali Ozcan, Safiye Aktas, Faize Yuksel, Serife Medeni Solmaz, Gokmen Omur Sevindik, Abdullah Katgi, Ozden Piskin, Bulent Undar

    • A primary multiple myeloma cell culture and a co-culture with host Treg cells were established.
    • The interaction between Treg cell expansion and the anti-tumor effect of bortezomib was revealed.
    • Treg cells had varied effects on bortezomib, increasing, decreasing, or not changing its anti-tumor effect on multiple myeloma.
    • Treg cells increased the ex vivo anti-tumor effect of bortezomib in patients who did not develop bortezomib resistance in vivo.
    • Treg cells decreased the ex vivo anti-tumor effect of bortezomib in patients who developed bortezomib resistance in vivo. 

    Expression of the MOZ-TIF2 oncoprotein in mice represses senescence
    Anne Largeot, Flor Maria Perez-Campo, Elli Marinopoulou, Michael Lie-a-Ling, Valerie Kouskoff, Georges Lacaud

    • MOZ-TIF2 transformation is associated with inhibition of senescence through repression of the CDKN2A locus.
    • MOZ histone acetyltransferase activity of MOZ-TIF2 is required for the inhibition of senescence.
    • MOZ-TIF2 acts on the p53 apoptosis-versus-senescence balance.

    Erythroblast enucleation is a dynein-dependent process
    Isuzu Kobayashi, Kumi Ubukawa, Kotomi Sugawara, Ken Asanuma, Yong-Mei Guo, Junsuke Yamashita, Naoto Takahashi, Kenichi Sawada, Wataru Nunomura

    • Dynein was expressed during terminal differentiation of human erythroblasts.
    • Inhibition of dynein blocked the cell proliferation of colony-forming units–erythroid and enucleation.
    • Dynein was distributed in aggregates on the periphery of the nucleus in erythroblasts.
    • Cell polarization was blocked in the dynein inhibitor-treated erythroblasts.
    • Enucleation of human erythroblasts requires cell polarization by dynein.

    Mobilization of hematopoietic stem cells with highest self-renewal by G-CSF precedes clonogenic cell mobilization peak
    Ingrid G. Winkler, Eliza Wiercinska, Valerie Barbier, Bianca Nowlan, Halvard Bonig, Jean-Pierre Levesque 

    • After 2 days of administration of granulocyte colony-stimulating factor, mouse blood contains high levels of serially reconstituting hematopoietic stem cells.
    • In mice, mobilization of serially reconstituting hematopoietic stem cells precedes that of clonogenic progenitors.
    • In half of healthy volunteers tested, after 2 days of administration of granulocyte colony-stimulating factor, mobilized CD34+ cells are enriched in CD34+CD38− primitive progenitors.

     

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    Inside this Issue: February

    Posted By Connections Editor, Thursday, February 18, 2016

    Hematopoietic niches, erythropoiesis and anemia of chronic infection
    Ana Cordeiro Gomes, Maria Salomé Gomes


    Anemia is a significant co-morbidity of chronic infections, as well as other inflammatory diseases. Anemia of chronic infection results from defective bone marrow erythropoiesis. Although the limitation of iron availability has been considered a key factor, the exact mechanisms underlying blockade in erythroid generation during infection are not fully understood. Erythropoiesis is a tightly regulated process that is very sensitive to environmental changes. During the last decade, the importance of the bone marrow hematopoietic niche has been progressively acknowledged.

    How hematopoietic stem/progenitors and their niche sense and respond to infectious stress

    Hiroshi Kobayashi, Toshio Suda, Keiyo Takubo


    Hematopoietic stem/progenitor cells (HSPCs) play important roles in fighting systemic infection as they supply immune cells in a demand-adapted manner. Various mechanisms govern HSPC responses to infection, including cytokine signaling, niche function, and direct sensing of pathogen-derived molecules by HSPCs themselves. Here we review recent advances in our understanding of HSPC responses to infection and also consider newly identified STING-mediated machinery recognizing bacteria-derived cyclic dinucleotides.

    Hematopoiesis “awakens”: Evolving technologies, the force behind them

    Eugenia Flores-Figueroa, Marieke Essers, Teresa V. Bowman


    Amid the beauty of the Kyoto countryside, leaders in the field of hematology met at the 44th annual International Society for Experimental Hematology (ISEH) meeting in late September 2015. Led by ISEH President Paul Frenette and President-Elect David Traver, the meeting covered many aspects of hematopoiesis with a focus on technology. At the meeting, it became clear that the future of hematology is being shaped by innovations in single-cell “omics” and imaging approaches that will provide answers to age-old questions on cellular identity.

    Kinetics of lentiviral vector transduction in human CD34+ cells

    Naoya Uchida, Rashidah Green, Josiah Ballantine, Luke P. Skala, Matthew M. Hsieh, John F. Tisdale


    Cellular labile iron, which contains chelatable redox-active Fe2+, has been implicated in iron-mediated cellular toxicity leading to multiple organ dysfunction. Iron homeostasis is controlled by monocytes/macrophages through their iron recycling and storage capacities. Furthermore, iron sequestration by monocytes/macrophages is regulated by pro-inflammatory cytokines including interleukin-1, highlighting the importance of these cells in the crosstalk between inflammation and iron homeostasis. However, a role for cellular labile iron in monocyte/macrophage-mediated inflammatory responses has not been defined.

    Activation of the NLRP3 inflammasome by cellular labile iron

    Kyohei Nakamura, Toru Kawakami, Naoki Yamamoto, Miyu Tomizawa, Tohru Fujiwara, Tomonori Ishii, Hideo Harigae, Kouetsu Ogasawara


    Cellular labile iron, which contains chelatable redox-active Fe2+, has been implicated in iron-mediated cellular toxicity leading to multiple organ dysfunction. Iron homeostasis is controlled by monocytes/macrophages through their iron recycling and storage capacities. Furthermore, iron sequestration by monocytes/macrophages is regulated by pro-inflammatory cytokines including interleukin-1, highlighting the importance of these cells in the crosstalk between inflammation and iron homeostasis. However, a role for cellular labile iron in monocyte/macrophage-mediated inflammatory responses has not been defined.

    Effects and mechanism of arsenic trioxide in combination with rmhTRAIL in multiple myeloma

    Hebing Zhou, Jinqing Li, Yuan Jian, Tingting Chen, Haiteng Deng, Juan Zhang, Hui Zeng, Zhijuan Shan, Wenming Chen


    The anti-tumor potential of arsenic trioxide (ATO) and recombinant mutant human TRAIL (rmhTRAIL) has been confirmed in various kinds of tumors. However, the effects and mechanism of the two drugs in combination in multiple myeloma (MM) have not been established. In this study, we evaluated the proliferation inhibition and apoptosis induction effects of ATO and rmhTRAIL as single agents and in combination on the MM cell lines RPMI8226 and U266. Then, we used high-performance liquid chromatography and mass spectrometry to find differentially expressed proteins before and after drug treatment and to analyze the mechanism underlying the effect of ATO and rmhTRAIL on MM cells.

    Bleeding the laboratory mouse: Not all methods are equal
    Jonathan Hoggatt, Amber F. Hoggatt, Tiffany A. Tate, Jeffrey Fortman, Louis M. Pelus


    The laboratory mouse is the model most frequently used in hematologic studies and assessment of blood parameters across a broad range of disciplines. Often, analysis of blood occurs in a nonterminal manner. However, the small body size of the mouse limits collection based on volume, frequency, and accessible sites. Commonly used sites in the mouse include the retro-orbital sinus, facial vein, tail vein, saphenous vein, and heart. The method of blood acquisition varies considerably across laboratories and is often not reported in detail.

    Immunoregulatory effects on T lymphocytes by human mesenchymal stromal cells isolated from bone marrow, amniotic fluid, and placenta

    Katia Mareschi, Sara Castiglia, Fiorella Sanavio, Deborah Rustichelli, Michela Muraro, Davide Defedele, Massimiliano Bergallo, Franca Fagioli


    Mesenchymal stromal cells (MSCs) are a promising tool in cell therapies because of their multipotent, bystander, and immunomodulatory properties. Although bone marrow represents the main source of MSCs, there remains a need to identify a stem cell source that is safe and easily accessible and yields large numbers of cells without provoking debates over ethics. In this study, MSCs isolated from amniotic fluid and placenta were compared with bone marrow MSCs. Their immunomodulatory properties were studied in total activated T cells (peripheral blood mononuclear cells) stimulated with phytohemagglutinin (PHA-PBMCs).

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